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A study done by Dr. Andrew Creer (2007 South Dakota State University) showed Optygen reduces cortisol levels (the primary stress hormone) by 26%. Researchers concluded that Optygen is beneficial for trained athletes undergoing high training volumes because it decreases baseline stress and improves training sessions. Cortisol is a catabolic (breakdown) hormone that the body produces when under stress, such as after hours of running. Chronically elevated cortisol levels can prevent an athlete from recovering, increase the risks of overtraining and supress the immune system.

Another study done by Dr. Karlton Larson (2007 Luther College) showed endurance athletes who used Optygen increased the time to lactate threshold by 42%. Results of this study show Optygen is effective in reducing lactic acid production and delaying onset of lactate threshold (the exercise intensity at which lactic acid starts to accumulate in the blood stream), therefore improving endurance.